DGE and Gene Ontology Analysis of Superior Mesenteric Ganglion during Inflammation

SMG of infected and naive Snap25CreINTACT mice were isolated. Nuclei isolation was performed and neuronal nuclei were sort-purified (GFP+). Bulk RNA-Sequencing was done and the results are shownbelow.

1 DESeq2

1.1 Results

1.1.1 Nippo

DGE: SMG Nippo vs Naive
DESeq2 Analysis

1.1.2 DSS

DGE: SMG DSS vs Naive
DESeq2 Analysis

1.1.3 Th17

DGE: SMG Th17 vs Naive
DESeq2 Analysis


1.2 Volcano Plots





Gene Lists are filtered for Log2FoldChange: upregulated > 1.5 & downregulated < -1.5 and padj < 0.05 For Analysis, gene symbols were used, and ordered query was performed

2 GSEA

2.1 gProfiler Enrichment Analysis

2.1.1 Nippo


SMG Nippo vs Naive
gProfiler Analysis



2.1.2 DSS


SMG DSS vs Naive
gProfiler Analysis



2.1.3 Th17


SMG Th17 vs Naive
gProfiler Analysis




2.2 clusterProfiler Enrichment Analysis

2.2.1 Nippo


SMG Nippo vs Naive
clusterProfiler Analysis



2.2.2 DSS


SMG DSS vs Naive
clusterProfiler Analysis



2.2.3 Th17


SMG Th17 vs Naive
clusterProfiler Analysis




2.3 KEGG

2.3.1 Nippo

SMG Nippo vs Naive
KEGG Analysis

2.3.2 DSS

SMG DSS vs Naive
KEGG Analysis

2.3.3 Th17

SMG Th17 vs Naive
KEGG Analysis




2.4 WikiPathways

2.4.1 Nippo

SMG Nippo vs Naive
WikiPathways Analysis

2.4.2 DSS

SMG DSS vs Naive
WikiPathways Analysis

2.4.3 Th17

SMG Th17 vs Naive
WikiPathways Analysis


2.5 Reactome

2.5.1 Nippo

SMG Nippo vs Naive
Reactome Analysis

2.5.2 DSS

SMG DSS vs Naive
Reactome Analysis

2.5.3 Th17

SMG Th17 vs Naive
Reactome Analysis