DGE and Gene Ontology Analysis of the Enteric Nervous System during Inflammation

Muscularis of infected and naive Snap25CreINTACT mice was isolated. Nuclei isolation was performed and neuronal nuclei were sort-purified (GFP+). Bulk RNA-Sequencing was done and the results are shownbelow.

1 DESeq2

1.1 Results

1.1.1 Nippo

DGE: ENS Nippo vs Naive
DESeq2 Analysis

1.1.2 DSS

DGE: ENS DSS vs Naive
DESeq2 Analysis

1.1.3 Th17

DGE: ENS Th17 vs Naive
DESeq2 Analysis


1.2 Volcano Plots





Gene Lists are filtered for Log2FoldChange: upregulated > 1.5 & downregulated < -1.5 and padj < 0.05 For Analysis, gene symbols were used, and ordered query was performed

2 GSEA

2.1 gProfiler Enrichment Analysis

2.1.1 Nippo


ENS Nippo vs Naive
gProfiler Analysis



2.1.2 DSS


ENS DSS vs Naive
gProfiler Analysis



2.1.3 Th17


ENS Th17 vs Naive
gProfiler Analysis




2.2 clusterProfiler Enrichment Analysis

2.2.1 Nippo


ENS Nippo vs Naive
clusterProfiler Analysis



2.2.2 DSS


ENS DSS vs Naive
clusterProfiler Analysis



2.2.3 Th17


ENS Th17 vs Naive
clusterProfiler Analysis




2.3 KEGG

2.3.1 Nippo

ENS Nippo vs Naive
KEGG Analysis

2.3.2 DSS

ENS DSS vs Naive
KEGG Analysis

2.3.3 Th17

ENS Th17 vs Naive
KEGG Analysis




2.4 WikiPathways

2.4.1 Nippo

ENS Nippo vs Naive
WikiPathways Analysis

2.4.2 DSS

ENS DSS vs Naive
WikiPathways Analysis

2.4.3 Th17

ENS Th17 vs Naive
WikiPathways Analysis


2.5 Reactome

2.5.1 Nippo

ENS Nippo vs Naive
Reactome Analysis

2.5.2 DSS

ENS DSS vs Naive
Reactome Analysis

2.5.3 Th17

ENS Th17 vs Naive
Reactome Analysis