DGE and Gene Ontology Analysis of Dorsal Root Ganglia during Inflammation

DRGs of infected and naive Snap25CreINTACT mice were isolated. Nuclei isolation was performed and neuronal nuclei were sort-purified (GFP+). Bulk RNA-Sequencing was done and the results are shownbelow.

1 DESeq2

1.1 Results

1.1.1 Nippo

DGE: DRG Nippo vs Naive
DESeq2 Analysis

1.1.2 DSS

DGE: DRG DSS vs Naive
DESeq2 Analysis

1.1.3 Th17

DGE: DRG Th17 vs Naive
DESeq2 Analysis


1.2 Volcano Plots





Gene Lists are filtered for Log2FoldChange: upregulated > 1.5 & downregulated < -1.5 and padj < 0.05 For Analysis, gene symbols were used, and ordered query was performed

2 GSEA

2.1 gProfiler Enrichment Analysis

2.1.1 Nippo


DRG Nippo vs Naive
gProfiler Analysis



2.1.2 DSS


DRG DSS vs Naive
gProfiler Analysis



2.1.3 Th17


DRG Th17 vs Naive
gProfiler Analysis




2.2 clusterProfiler Enrichment Analysis

2.2.1 Nippo


DRG Nippo vs Naive
clusterProfiler Analysis



2.2.2 DSS


DRG DSS vs Naive
clusterProfiler Analysis



2.2.3 Th17


DRG Th17 vs Naive
clusterProfiler Analysis




2.3 KEGG

2.3.1 Nippo

DRG Nippo vs Naive
KEGG Analysis

2.3.2 DSS

DRG DSS vs Naive
KEGG Analysis

2.3.3 Th17

DRG Th17 vs Naive
KEGG Analysis




2.4 WikiPathways

2.4.1 Nippo

DRG Nippo vs Naive
WikiPathways Analysis

2.4.2 DSS

DRG DSS vs Naive
WikiPathways Analysis

2.4.3 Th17

DRG Th17 vs Naive
WikiPathways Analysis


2.5 Reactome

2.5.1 Nippo

DRG Nippo vs Naive
Reactome Analysis

2.5.2 DSS

DRG DSS vs Naive
Reactome Analysis

2.5.3 Th17

DRG Th17 vs Naive
Reactome Analysis